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Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Synthetic, Human)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP-GFP
Plasmid#73579PurposeLentivirus expression of mOC-STAMPDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pEGFP C3
Plasmid#62960Purposefull length human Mucolipin-1 cloned into pEGFP C3DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsEGFPExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-hsFlt3L
Plasmid#25074DepositorInserthuman soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 WT
Plasmid#53468DepositorInsertRab05 (Rab5 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorUseTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg1
Plasmid#170330PurposeExpresses RORg isoform 1 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 1 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL728
Plasmid#140398PurposeExpresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoterDepositorInsertT7-csoO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL716
Plasmid#140386PurposeExpresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoterDepositorInsertT7-mphO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL703
Plasmid#140373PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoterDepositorInsertT7-2BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PH Akt-Venus
Plasmid#85223PurposeVenus tagged PH domain of Akt for use as a PIP3 sensorDepositorInsertPH AKT(1-148) (AKT1 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBG-PELP1
Plasmid#65209PurposeFull length mammalian expression vector for hPELP1DepositorInsertPELP1 (PELP1 Human)
UseTagsGSTExpressionMammalianMutationPromoterEF1aAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL724
Plasmid#140394PurposeExpresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoterDepositorInsertT7-hucO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL726
Plasmid#140396PurposeExpresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoterDepositorInsertT7-smtO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds
Plasmid#119075PurposeZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporterDepositorInsertubb:Com-KRAB-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 WT
Plasmid#44829DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL704
Plasmid#140374PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoterDepositorInsertT7-4BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL705
Plasmid#140375PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoterDepositorInsertT7-5BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp dsRed Rab5 CA
Plasmid#53467DepositorInsertRab05 (Rab5 Fly)
UseTagsdsRedExpressionInsectMutationQ88LPromoterGAL4Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBL729
Plasmid#140399PurposeExpresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoterDepositorInsertT7-cadO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab5 DN
Plasmid#44823DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS43N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL718
Plasmid#140388PurposeExpresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoterDepositorInsertT7-mobO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus-G protein Gamma 5
Plasmid#42200DepositorInsertGamma 5 (Gng5 Rat)
UseTagsVenus(1-155)ExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg2
Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 CA
Plasmid#44825DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ67L (constitutive active)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab5 DN
Plasmid#37694DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS43N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 CA
Plasmid#37702DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ88L (constitutive active)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1-3, aa 1-178)
Plasmid#66185PurposeExpresses TRABID NZF1-3 (aa 1-178) in E. coliDepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-178 of TRABIDPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 DN
Plasmid#44826DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationT22N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF Itk K390R myc
Plasmid#20707DepositorInsertItk K390R (Itk Mouse)
UseTagsmycExpressionMammalianMutationKinase Dead. K390R.PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJBL712
Plasmid#140382PurposeExpresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoterDepositorInsertT7-otrO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 CA
Plasmid#37707DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 DN
Plasmid#37706DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 CA
Plasmid#37698DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ71L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 DN
Plasmid#37697DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab7 CA
Plasmid#37704DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 DN
Plasmid#58448Purposeexpression of drosophila YFP-Rab6 dominant negative mutantDepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 WT
Plasmid#58447Purposeexpression of drosophila YFP-Rab6DepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab1 DN
Plasmid#37682DepositorInsertRab1 (Rab1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS25N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab7 DN
Plasmid#37703DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Itk TEC
Plasmid#20711DepositorInsertItk TEC (Itk Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 97-174PromoterAvailable sinceMarch 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT
Plasmid#187652PurposeContains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.DepositorInsertsHaloTag donor sequence
miRFP670
UseAAV and CRISPRTagsN/AExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL714
Plasmid#140384PurposeExpresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoterDepositorInsertT7-ctcO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL710
Plasmid#140380PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 14BP dowsntream from the promoterDepositorInsertT7-14BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only