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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,854 results for: 623

Showing: 801 - 820 of 2854 results

  2. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Use
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  3. pX459-puro-hBRAF-1

    Plasmid
    #185365
    Purpose
    For mammalian expression of guide RNA: caccgACAACAGTTATTGGAATCTC that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185365-plasmid-map-sequence-id-363594

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  4. pX459-puro-hBRAF-2

    Plasmid
    #185366
    Purpose
    For mammalian expression of guide RNA: caccgAAAATCCCACAGATGTGGCA that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185366-plasmid-map-sequence-id-363457

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  5. 5JJZ (CDYL2)

    Plasmid
    #101876
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDYL2 (CDYL2 Human)
    Use
    Tags
    6x His and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101876-plasmid-map-sequence-id-194525

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  11. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  14. KLHL7

    Plasmid
    #39023
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL7 (KLHL7 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. INPP5EA

    Plasmid
    #42478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INPP5EA (INPP5E Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pLEX307-ACE2-puro

    Plasmid
    #158448
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158448-plasmid-map-sequence-id-308163

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  19. pLEX307-ACE2-blast

    Plasmid
    #158449
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158449-plasmid-map-sequence-id-434046

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  20. pDONR221-ACE2

    Plasmid
    #158438
    Purpose
    Gateway Cloning compatible entry vector for the human ACE2 gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158438-plasmid-map-sequence-id-308248

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Showing: 801 - 820 of 2854 results