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We narrowed to 8,864 results for: LIC

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Showing: 801 - 820 of 8864 results

  2. pEU3-NII-GLICNot-C-BIOT

    Plasmid
    #140186
    Purpose
    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site
    Depositor
    Tamás Mészáros
    Publication
    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do
    Type
    Empty backbone
    Use
    Expression vector for a cell-free system
    Tags
    Biotinylation and GST
    Promoter
    T7
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140186-plasmid-map-sequence-id-300441

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  9. gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218446
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-Vh-hIgG1-CH6
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218446-plasmid-map-sequence-id-434499

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  10. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid
    #208961
    Purpose
    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208961-plasmid-map-sequence-id-415791

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  14. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  15. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  16. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid
    #208943
    Purpose
    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208943-plasmid-map-sequence-id-415668

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  19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  20. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid
    #246436
    Purpose
    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    Tags
    GFP N-terminal sequence (M1 to Q95), Hemi Intron …
    Expression
    Mammalian
    Mutation
    GFP N-terminal sequence M1 to Glutamine 95 append…
    Promoter
    CMV and U1a
    Available since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246436-plasmid-map-sequence-id-490914

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Showing: 801 - 820 of 8864 results