We narrowed to 14,442 results for: NTS

1. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 783 pCScherryActMEK

   Plasmid

   #31880

   Depositor

   Nathan Lawson

   Article

   Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Expression vector

   Tags

   mCherry

   Mutation

   Ser218Glu Ser222Asp; activated MEKK

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLVX-Puro-TDP-43-A90V

   Plasmid

   #133754

   Purpose

   Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed alanine 90 to valine

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HiBiT-FZD3

   Plasmid

   #195841

   Purpose

   Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD3 (FZD3 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   I292V and V495I

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Puro-TDP-43-P112H

   Plasmid

   #133760

   Purpose

   Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed proline 112 to histidine.

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM-60-NusA-TEV-HsRCK_296-472_E

   Plasmid

   #146209

   Purpose

   Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

   Plasmid

   #166791

   Purpose

   Mammalian cell expression vector for His-tagged DPP9S

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   HIS-TEV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1053 pBabe puroL Akt K179M T308A S473A

   Plasmid

   #9013

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV.U6.gDNMT1.Cas9-T2A-GFP

    Plasmid

    #170362

    Purpose

    Plasmid used for Crispr/cas9 based disruption of human DNMT1.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGT6471

    Plasmid

    #219428

    Purpose

    Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
    

    Use

    Moclo compatible level 0 module

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3_T7-mScarlet3-KRas6R

    Plasmid

    #225930

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas6R
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 10_T7-SP-CD8tm-mScarlet3

    Plasmid

    #225937

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    SP-CD8tm-mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Venus-MDGA2

    Plasmid

    #82544

    Purpose

    Overexpression vector for Venus-MDGA2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA2 (Mdga2 Rat)
    

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Promoter

    chicken beta actin

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRC/CMV-JAK1K908E-VSV

    Plasmid

    #139357

    Purpose

    Expression of kinase-dead JAK1-K908E protein

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    K908E

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUPI hT5-S322-Cyp

    Plasmid

    #80282

    Purpose

    Expresses human TRIM5 CypA fusion

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion (TRIM5, PPIA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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