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pEF myc-SHP2(Q510E)-TurboID

Plasmid

#245250

Purpose

Proximity-labeling proteomics with SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

TurboID

Expression

Mammalian

Mutation

Q510E

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA c-Src KD

Plasmid

#245256

Purpose

Expression of the kinase domain of c-Src in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

Kinase domain of c-Src

Expression

Yeast

Mutation

wild-type

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T52S)-TurboID

Plasmid

#245244

Purpose

Proximity-labeling proteomics with SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

TurboID

Expression

Mammalian

Mutation

T52S

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK157

Plasmid

#242908

Purpose

Constitutive expression of dCas9, MCP-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Inserts

MCP-SYNZIP6
αNTD-SYNZIP5

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK147.02

Plasmid

#242909

Purpose

Constitutive expression of dCas9, PspF-λN22, and boxB scRNA J102

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA boxB J102

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK692

Plasmid

#242910

Purpose

Constitutive expression of dCas9 and αNTD-MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

αNTD-MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK321

Plasmid

#242912

Purpose

aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-H2B-eGFP-SV40-Hygro

Plasmid

#240801

Purpose

Constitutive expression of H2B-eGFP

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

H2B-eGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239746

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

Plasmid

#239742

Purpose

Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

Plasmid

#239743

Purpose

Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239744

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239745

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239747

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge