166,224 results

1. pSBbi-BB

   Plasmid

   #60521

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   Eric Lander, David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. STING-miRFP680

   Plasmid

   #200940

   Purpose

   STING fused with miRFP680 for live cell imaging

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   STING (STING1 Human)
   miRFP680
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP1-N1-Dsup

   Plasmid

   #90020

   Purpose

   To express GFP-tagged Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-Gsdmd-NT

   Plasmid

   #80951

   Purpose

   Expresses Gsdmd-NT in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT (Gsdmd Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmEmerald-ERGIC-53

   Plasmid

   #170717

   Purpose

   Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   ERGIC-53 (LMAN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLminP_Luc2P_RE22

   Plasmid

   #90364

   Purpose

   STAT6 - Stat6 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT6

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIN40C.SFFV.Luciferase.IRES.GFP

   Plasmid

   #169308

   Purpose

   Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

   Plasmid

   #156440

   Purpose

   For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SA1 (Stag1 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-PKCA WT

    Plasmid

    #195202

    Purpose

    Mammalian expression vector for GFP tagged PKCA.

    Depositor

    David Bryant, Peter Parker

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Expression

    Mammalian

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Expression

    Mammalian

    Promoter

    pTRE3G

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mito-Citron1

    Plasmid

    #134305

    Purpose

    mitochondria-targeted direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3083

    Plasmid

    #144559

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOSL2 (FOSL2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_human CDK1

    Plasmid

    #118597

    Purpose

    One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    guide RNA against human CDK1
    

    Use

    CRISPR

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBHRSF184

    Plasmid

    #89482

    Purpose

    Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

    Depositor

    Hideo Iwai

    Article

    Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-CAG-hCas3

    Plasmid

    #134920

    Purpose

    Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas3 with bpNLS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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