173,520 results

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pSpCas9_T2A_mCherry sgAAVS1

Plasmid

#192480

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPM1wt-C1

Plasmid

#131818

Purpose

Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLGB36

Plasmid

#135621

Purpose

Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

Depositor

Laurie Comstock

Article

Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

Insert

bfe3

Use

Bacteroides suicide vector with inducible counter…

Tags

signal sequence for periplasmic targeting

Promoter

Bacteroides promoter regualted by TetR transcript…

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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P362-FBP_12

Plasmid

#139703

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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hIRE1a.pcD

Plasmid

#21892

Depositor

Randal Kaufman

Insert

IRE1a (ERN1 Human)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-SpRY (pBM1571)

Plasmid

#170105

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

Promoter

CMV

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 delta

Plasmid

#20785

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

pDCNA3 Flag p38 delta (Mapk13 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe N-Ras 61K

Plasmid

#12543

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

NRas 61K (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

61K constitutively activated.

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB2

Plasmid

#197361

Purpose

Encoding human ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP

Plasmid

#48885

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI, sfGFP, and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-GDH

Plasmid

#108954

Purpose

heterologous expression of GDH of B. megaterium in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Glucose dehydrogenase IV (GDH IV)

Tags

His6-Tag

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP8

Plasmid

#45912

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pMXsIP GFP-VPS33A

Plasmid

#67022

Purpose

human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
CT752

Plasmid

#233658

Purpose

Flp GFP Reporter; FRT-STOP-FRT-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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