164,223 results

1. pcDNA3-EGFP-Rac1-Q61L

   Plasmid

   #12981

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMal-T-Avi-His/BirA

   Plasmid

   #102962

   Purpose

   Vector for in vivo biotinylation of a fusion protein in bacteria.

   Depositor

   Tonia Rex

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1 gfaABC1D-cyto-GCaMP6f

   Plasmid

   #52925

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Baljit Khakh

   Article

   Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

   Has Service

   AAV5

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AALN-BE4max

   Plasmid

   #138161

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(AALN)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTHSSe_60

   Plasmid

   #109254

   Purpose

   TALEsp2 stabilized promoter driving GFP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TALEsp2 stabilized promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-AktAR2

   Plasmid

   #64932

   Purpose

   Enhanced FRET-based biosensor for monitoring Akt activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   AktAR2
   

   Use

   Tags

   Cerulean3 and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-CDK4

   Plasmid

   #116724

   Purpose

   Lentiviral expression of CDK4

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ac5-STABLE2-puro

    Plasmid

    #208032

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXW117Ptet2-gfp

    Plasmid

    #160818

    Purpose

    aTc-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23117-30tetR-t-Ptet2-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23117, Ptet2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTS-hFluc

    Plasmid

    #177716

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    Mitochondrial Targeting Sequence MTS SS

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Neo-Bam APC 1-1309

    Plasmid

    #16509

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon 1309 truncation

    Promoter

    Available Since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-­RAGE

    Plasmid

    #71435

    Purpose

    mammalian expression of human RAGE

    Depositor

    Henri Huttunen

    Article

    Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24

    Insert

    RAGE (AGER Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

    Plasmid

    #200400

    Purpose

    Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

    Depositor

    Daniel Kronauer

    Article

    Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

    Inserts

    Discosoma red fluorescent protein
    Q factor 2
    GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBNde(min)

    Plasmid

    #15125

    Depositor

    Franco Pagani

    Article

    Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

    Insert

    alpha-globin EDB fibronectin minigene (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    alpha-globin

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CL20_mEGFP_EWSR1_ERG

    Plasmid

    #205819

    Purpose

    Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EWSR1_ERG (EWSR1, ERG Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Multi-frame tag

    Plasmid

    #128610

    Purpose

    In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

    Depositor

    Tim Stasevich

    Article

    Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

    Insert

    MF tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge