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pCL0981

Plasmid

#252427

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 47-102 only

Promoter

CMV

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316 Gal1p1

Plasmid

#242951

Purpose

Backbone plasmid for subcloning

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

Gal_promoter-cycE terminator

Tags

Myc-tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316 Gal1p1 IpgA

Plasmid

#242952

Purpose

Expression of IpgA in yeast cells

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IpgA (IcsB Chaperone)

Tags

Myc

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2

Plasmid

#242948

Purpose

Backbone plasmid for IcsB and homologs subcloning

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

Backbone plasmid for subcloning

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2 IcsB

Plasmid

#242949

Purpose

Expression of IcsB in yeast

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IcsB

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2 H8

Plasmid

#242950

Purpose

Expression of homolog H8 in yest cells

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IcsB Homolog H8

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0918

Plasmid

#252411

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-46 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0946

Plasmid

#252416

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-20 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL400_TETRIS-v3_empty

Plasmid

#251477

Purpose

TETRIS (transposable element to test RNA’s effect on transcription in cis)

Depositor

Mauro Calabrese

Article

Trotman et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK007

Plasmid

#245126

Purpose

Control plasmid based on pAF259 (Addgene #105494). Contains a promoterless luciferase-encoding bitluc.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Bitlucopt

Expression

Bacterial

Promoter

None

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab676-HBEGF-2a-mWasabi-WPRE

Plasmid

#249029

Purpose

This plasmid expresses the mWasabi flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mWasabi

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab639-HBEGF-2a-mRuby3-WPRE

Plasmid

#249030

Purpose

This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mRuby3

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab672-HBEGF-2a-mTagBFP2-WPRE

Plasmid

#249031

Purpose

This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mTagBFP2

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab821-pcDNA3.1-HALO-3xFLAG

Plasmid

#249007

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

HALO

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_Puro_donor

Plasmid

#246401

Purpose

PHF19 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_KO_Puro_donor

Plasmid

#246393

Purpose

MTF2 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMITO-mTFP1

Plasmid

#244030

Purpose

anchoring mTFP1 to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

CISD1-mTFP1

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_HTC3xF_BSD_donor

Plasmid

#246391

Purpose

MTF2 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_KO_Puro_donor

Plasmid

#246397

Purpose

PHF1 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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