We narrowed to 9,182 results for: Pol

1. pJEP221-AAV-RSV-GFP-pA

   Plasmid

   #62919

   Purpose

   AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   AAV

   Promoter

   Rous Sarcoma Virus(RSV)

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Promoterless TP10

   Plasmid

   #165787

   Purpose

   Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Promoterless T10
   

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACEBac1-Q22MS1-2xStrep

   Plasmid

   #170314

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22MS1
   

   Tags

   Strep-Tag II

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. IBMc393

   Plasmid

   #161953

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB4A3-T-PT7-B30-mScarlet-I-3T
   

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myo 9 Avi+Flag

   Plasmid

   #134969

   Purpose

   Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

   Depositor

   Martin Bähler

   Article

   Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

   Insert

   Myo 9 (hum-7 Nematode)
   

   Tags

   Avi and Flag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-T3-HypaCAS9-pA

   Plasmid

   #131467

   Purpose

   Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

   Insert

   Codon optimized HypaCas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS-BacA-21222-FLP-3xFLAG

   Plasmid

   #109165

   Purpose

   Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

   Insert

   Flp
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBac(3xP3-gTc’v; Valcyrie.LR)

   Plasmid

   #119066

   Purpose

   Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2019 Jan 4;146(1). pii: dev

   Inserts

   pBacL
   Tc-U6a-Lox.L
   Tc-hsp68 Promoter
   NLS-Cas9
   Tc-hsp68 3'UTR
   Tc-U6B-Lox.R
   SV40polyA
   Tc-vermilion
   3xP3 TATA
   pBacR
   

   Expression

   Insect

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC111

   Plasmid

   #118906

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
   

   Tags

   LPETGg, HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE80L TriCatcher-MMP

    Plasmid

    #112632

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3R112C/T180C-GG

    Plasmid

    #85093

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1G108C-GG

    Plasmid

    #85092

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Glycine 108 to Cysteine

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIB-HISX6-TEV-RSP4

    Plasmid

    #176693

    Purpose

    Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP4
    

    Tags

    HISX6

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIB-RSP5

    Plasmid

    #176694

    Purpose

    Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP5 (RSP5 Chlamydomonas reinhardtii)
    

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pB_tetO7_sfGFP

    Plasmid

    #196616

    Purpose

    Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    Superfolder GFP
    

    Expression

    Yeast

    Promoter

    tetO7 promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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