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T7-10-QUAS-GFP_ColE1

Plasmid

#171657

Purpose

QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-10-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-15-QUAS-GFP_ColE1

Plasmid

#171658

Purpose

QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-15-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-TetO-QF_T7-LacO-TetR_p15A

Plasmid

#171659

Purpose

Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-TetR-T7 Stop

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-0-QUAS-GFP_ColE1

Plasmid

#171650

Purpose

QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-0-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-CcdB_ColE1

Plasmid

#171652

Purpose

QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

CcdB-ssrA

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-5-QUAS-GFP_ColE1

Plasmid

#171656

Purpose

QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-5-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS538

Plasmid

#188881

Purpose

Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Cas9

Use

Gateway Cloning

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6730

Plasmid

#139155

Purpose

PapFAB45 - TARGET3 - MevT - MBIS

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

MevT-MBIS

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6731

Plasmid

#139156

Purpose

Pstabilized - TARGET8 - MevT - MBIS

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

MevT-MBIS

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6691

Plasmid

#139157

Purpose

PapFAB45 - apFAB682 - EsaI - LuxR

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

EsaI - LuxR

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6656

Plasmid

#139133

Purpose

PgntK - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6657

Plasmid

#139134

Purpose

PompF - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6658

Plasmid

#139135

Purpose

PyeeF - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL6659

Plasmid

#139136

Purpose

PompT - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6660

Plasmid

#139137

Purpose

PmetN - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6661

Plasmid

#139138

Purpose

Pb1762 - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL6662

Plasmid

#139139

Purpose

PcarA - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL6663

Plasmid

#139140

Purpose

PfadL - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL6664

Plasmid

#139141

Purpose

PfecA - TARGET8 - mCherry

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

mCherry

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge