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pMRXIP-GFP-5K4Q

Plasmid

#221740

Purpose

Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Publication

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP580

Plasmid

#222959

Purpose

Expresses R141S K142S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Publication

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine and Lysine 142 to …

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP579

Plasmid

#222958

Purpose

Expresses R36S K51S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Publication

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine and Lysine 51 to Se…

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-AC17-5c-frGFP

Plasmid

#217525

Purpose

AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Publication

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

AC17-5c riboswitch-regulated folding reporter GFP

Use

In vitro transcription-translation

Promoter

T7

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIBY55

Plasmid

#213706

Purpose

Express the affinity sorting tag TST-EGFP- GPIBY55.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPICEAM7

Plasmid

#213705

Purpose

Express the affinity sorting tag TST-EGFP- GPICEAM7.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIDAF

Plasmid

#213704

Purpose

Express the affinity sorting tag TST-EGFP- GPIDAF.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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