We narrowed to 22,434 results for: ESC;

1. pET11a-N-DiB2

   Plasmid

   #168472

   Purpose

   N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

   Insert

   N-fragment of DiB2
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1026L=U6-3-gRNA#1[Pol-γ35]

   Plasmid

   #159674

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#1[Pol-γ35]
   

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1026M=U6-3-gRNA#2[Pol-γ35]

   Plasmid

   #159675

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#2[Pol-γ35]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNL1141

   Plasmid

   #160867

   Purpose

   PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

   Depositor

   John Dueber

   Article

   Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

   Insert

   Coniferyl alcohol acyltransferase
   

   Use

   Synthetic Biology

   Tags

   mRuby2

   Expression

   Bacterial

   Promoter

   PJ23110

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLNL1127

   Plasmid

   #160859

   Purpose

   PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

   Depositor

   John Dueber

   Article

   Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

   Insert

   Acetyl CoA geraniol/citronellol acetyltransferase
   

   Use

   Synthetic Biology

   Tags

   mRuby2

   Expression

   Bacterial

   Promoter

   PJ23110

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVD_01

   Plasmid

   #149606

   Purpose

   pTEF1-VanR inserted in pRS416 backbone

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   VanR
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

   Plasmid

   #162814

   Purpose

   STELLA plasmid for amber suppression of C-terminally labelled SMIM38

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   SMIM38(C-TAG)-Intein-DD
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   SMIM38(Ochre52,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

   Plasmid

   #162803

   Purpose

   Control STELLA destination plasmid for N-terminally labelled proteins

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub(G75,76A)-BsmBI
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   Ubiquitin (G75,76A)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4x7SKPylT_EF1_Ub-*SMIM38

   Plasmid

   #162808

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled SMIM38

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)SMIM38
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   SMIM38 (Met1, Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4x7SKPylT_EF1_Ub-*PIGBOS

    Plasmid

    #162809

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)PIGBOS
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    PIGBOS (Met1, Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 907F1

    Plasmid

    #160233

    Purpose

    FlightN-VanR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    VanR
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ414 - DANCER-2

    Plasmid

    #136386

    Purpose

    Expresses DANCER-2 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-2
    

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ414 - NERD-S

    Plasmid

    #136388

    Purpose

    Expresses NERD-S static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-S
    

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ414 - NERD-C

    Plasmid

    #136389

    Purpose

    Expresses NERD-C static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-C
    

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-F30L-V39I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG369-lyn11-redFAST

    Plasmid

    #160356

    Purpose

    Expresses Lyn11-redFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    lyn11-redFAST
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG361-lyn11-greenFAST

    Plasmid

    #160352

    Purpose

    Expresses Lyn11-greenFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    lyn11-greenFAST
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EcNusGNTD

    Plasmid

    #113123

    Purpose

    Expresses N-terminus of E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG
    

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 120-181

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIntegrase-AntiDsRed

    Plasmid

    #139677

    Purpose

    Cassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporter

    Depositor

    James Birchler

    Article

    Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

    Insert

    AntiDsRed Integrase
    

    Expression

    Plant

    Promoter

    Maize Ubiquitin

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZS039

    Plasmid

    #134233

    Purpose

    For in vitro transcription of Miniata CENP-A

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Miniata CENP-A n-terminus (nt 1-204, aa 1-68)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZS077

    Plasmid

    #134235

    Purpose

    Bacterial expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    GST-CENP-C
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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