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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

Plasmid

#237891

Purpose

Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

Plasmid

#237892

Purpose

Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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iSM_PPL2-IP

Plasmid

#235989

Purpose

Expresses iSM_PPL2 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_PPL2

Use

CRISPR

Expression

Mammalian

Mutation

replacement of 5 amino acids of Smac-Cas9 PL2 wit…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-N2-BE4-puro

Plasmid

#226583

Purpose

Express N2-BE4 base editor in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

N2-BE4

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM2_pGEX

Plasmid

#228799

Purpose

Recombinant expression of mouse MSI1 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_2xRRM_pGEX

Plasmid

#228800

Purpose

Recombinant expression of MSI2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM1_pGEX

Plasmid

#228801

Purpose

Recombinant expression of MSI2 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM1 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM2_pGEX

Plasmid

#228802

Purpose

Recombinant expression of MSI2 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_pGEX_noSBP

Plasmid

#228803

Purpose

Recombinant expression of UNK with mono-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt (UNK Human)

Tags

GST and HIS

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Swap_pGEX

Plasmid

#228804

Purpose

Recombinant expression of UNK Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6, 1-3 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Dual_pGEX

Plasmid

#228805

Purpose

Recombinant expression of UNK Dual with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6 x2 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_2xRRM_pGEX_Flag_noSBP

Plasmid

#228796

Purpose

Recombinant expression of mouse MSI1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 (Msi1 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_SWAP_2xRRM_pGEX

Plasmid

#228797

Purpose

Recombinant expression of mouse MSI1 Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2, RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM1_pGEX

Plasmid

#228798

Purpose

Recombinant expression of mouse MSI1 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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