We narrowed to 14,138 results for: flag

1. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Promoter

   TgTUB1 and Toxoplasma U6

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPORT-RREB1

   Plasmid

   #41145

   Depositor

   Joshua Mendell

   Article

   Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

   Insert

   RREB-1 (RREB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Promoter

   AtRPS5A

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-SS-345

   Plasmid

   #68785

   Purpose

   R->A Sensor pair 1 with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   C-Terminal Sensor 1 V2 R-->A mutant
   N-Terminal Sensor 1 V2 R-->A mutant
   mCherry
   

   Tags

   NLS, flag, and nls

   Expression

   Mammalian

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLSLGFP

   Plasmid

   #51494

   Purpose

   pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GFP
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP Cas

   Plasmid

   #50729

   Purpose

   mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cas (Bcar1 Rat)
   

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEntr-Tbx5flbio

   Plasmid

   #32968

   Depositor

   William Pu

   Article

   He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

   Insert

   Tbx5 (TBX5 Human)
   

   Use

   Gateway

   Tags

   flag-bio

   Available Since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCas9-P2A-BFP

   Plasmid

   #149447

   Purpose

   Expresses Cas9 and BFP in a lentiviral vector.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   P2A-BFP
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP2BA

   Plasmid

   #31369

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

   Insert

   Per2 (Per2 Mouse)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

10. TAL2065

    Plasmid

    #39435

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-39-Right
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2064

    Plasmid

    #39434

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-39-Left
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. BALF5-WT

    Plasmid

    #192454

    Purpose

    Expresses wild-type BALF5 (codon-optimized)

    Depositor

    Bill Sugden

    Article

    Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

    Insert

    BALF5 (WT) (BALF5 Epstein-Barr Virus)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LPCX Smad3

    Plasmid

    #12638

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCEV-G1-Ph

    Plasmid

    #46814

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Promoter

    TEF1 and PGK1

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSANG10-3F

    Plasmid

    #39264

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Bacterial expression, bacterial expression of re…

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEV-G2-Km

    Plasmid

    #46815

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Promoter

    TEF1 and PGK1

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1 hBRM

    Plasmid

    #1956

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BRM (SMARCA2 Human)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pKAR1

    Plasmid

    #23104

    Purpose

    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid

    Depositor

    Randy Poon

    Article

    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    TRE for cDNA; U6 for shRNA

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShuttle-FEN1mWT

    Plasmid

    #35032

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1mWT
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only