We narrowed to 14,587 results for: pET

1. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

   Plasmid

   #153440

   Purpose

   E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode A knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 191 and leucine 195 on XDocB were mutate…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 Mocr TEV co-transformation cloning vector (13K-O)

   Plasmid

   #48320

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Mocr

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 Mocr TEV co-transformation cloning vector (13S-O)

   Plasmid

   #48327

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Mocr

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

   Plasmid

   #96888

   Purpose

   expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

   Depositor

   Hening Lin

   Article

   Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

   Insert

   Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
   

   Use

   Tags

   FLAG-tagged

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-36, mutated lysine 351 to ar…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

   Plasmid

   #111398

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

   Plasmid

   #111399

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

   Plasmid

   #55209

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   10xAsn, 10xHis, MPB, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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