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KLHL31 pgk (1088)

Plasmid

#62031

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KLHL31 (Klhl31 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

Plasmid

#62579

Purpose

Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Promoter

EF1a

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-eGFP

Plasmid

#62518

Purpose

Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-mCherry

Plasmid

#222326

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ucp1-Cre-miR122

Plasmid

#228408

Purpose

Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

Depositor

Joerg Heeren

Article

Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

Inserts

cre recombinase with SV40 NLS
Cre recombinase with SV40 NLS

Use

AAV

Promoter

rat Ucp1 enhancer-promoter

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-'No'Mi-Red-T2A-Puro

Plasmid

#246642

Purpose

EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.

Depositor

Koen Breyne

Article

Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70

Insert

cleavable E-NoMi-Red

Use

Lentiviral

Tags

FLAG-Nanoluciferase and mCherry

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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