We narrowed to 118,280 results for: MPI;

1. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

   Plasmid

   #221449

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

   Plasmid

   #221450

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

   Plasmid

   #221451

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-PiGM-Iq(535/81, eGFP)

   Plasmid

   #221612

   Purpose

   AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

   Depositor

   John Lin

   Insert

   PiGM-Iq (535/81,eGFP)
   

   Use

   AAV

   Mutation

   RGS2 1-53 truncation

   Promoter

   Human Synapsin

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3_THADA_alltoneg0

   Plasmid

   #232372

   Purpose

   THADA enhancer, all sensitizing elements made into buffering Coordinator

   Depositor

   Joanna Wysocka

   Insert

   THADA (THADA Human)
   

   Use

   Luciferase

   Mutation

   THADA enhancer, all sensitizing elements made int…

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPMS-2016

   Plasmid

   #232290

   Purpose

   Protein Expression

   Depositor

   Jeremy Nathans

   Insert

   Apcdd1 (Apcdd1 Mouse)
   

   Tags

   8X His-Avi-1D4

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpec3'

   Plasmid

   #228548

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV_EF1a_mNeon

    Plasmid

    #212640

    Purpose

    Lentivirus vector to express mNeon

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    mNeon
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EGFP-SKL

    Plasmid

    #183727

    Purpose

    For exrpression of GFP targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Promoter

    UbiC

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid

    #218970

    Purpose

    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

    Depositor

    Benjamin Glick

    Insert

    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    moxGFP2

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-35aaNSP1noMSA11-NSP5(io)

    Plasmid

    #220827

    Purpose

    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length recoded NSP5 protein

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1noM-NSP5(io)
    

    Use

    Other

    Mutation

    the 35aa NSP1 lacks all Methionines

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuideSLC35C1-neo

    Plasmid

    #220868

    Purpose

    encodes CRISPR sgRNA targeting human SLC3C1

    Depositor

    Jennifer Kohler

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP

    Plasmid

    #220828

    Purpose

    Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-P2A-NSP5(io)-GS-smURFP
    

    Use

    Other

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-35aaNSP1noMSA11-NSP5(wt)

    Plasmid

    #220826

    Purpose

    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length SA11 NSP5 protein

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1noM-NSP5(wt)
    

    Use

    Other

    Mutation

    the 35aa NSP1 lacks all Methionines

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-35aaNSP1SA11-Green Lantern(wt)

    Plasmid

    #220821

    Purpose

    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-Green Latern(wt)
    

    Use

    Other

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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