We narrowed to 13,664 results for: Cre

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CCKBR-DuET

Plasmid

#213196

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCKBR (CCKBR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD18

Plasmid

#208270

Purpose

Production of ent-isokaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

ptmT2
osKSL6

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK6-P2A-Hygro_Barcode

Plasmid

#170224

Purpose

Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9749-dABE

Plasmid

#162998

Purpose

plasmid expressing ABE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab32a

Plasmid

#109689

Purpose

Multisite gateway vector for 3' tagging with rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11bb

Plasmid

#109597

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11bb (rab11bb Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-CD44b-mKate2

Plasmid

#109524

Purpose

Muscle specific zebrafish expression of mKate2 fused to CD44b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44b (cd44b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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vector plix 4.03

Plasmid

#121130

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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KO801: pMVP (L5-L4) Hygro-P2A

Plasmid

#121718

Purpose

pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Hygro-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL4_P2A_Hygro_Barcode

Plasmid

#120429

Purpose

Barcoded lentiviral vector to express ASCL4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL4 (ASCL4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLMB712

Plasmid

#83784

Purpose

Hesperetin biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 1

Plasmid

#99316

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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