We narrowed to 18,860 results for: REV;
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Plasmid#232101PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pDest17_Gcn4 Aro3+
Plasmid#231884PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro3+DepositorInsertGcn4 Aro3+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationD103Y, N112T, L113Y, S117F, K140RPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Aro2+
Plasmid#231883PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro2+DepositorInsertGcn4 Aro2+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationN116F, S117E, E119N, P129Q, T132YPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WTSLFD
Plasmid#231881PurposeBacterial expression of N-terminally 6His tagged Gcn4 WTSLFDDepositorInsertGcn4 WTSLFD
Tags6xHis, TEV cleavage siteExpressionBacterialMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 F108A+
Plasmid#231875PurposeBacterial expression of N-terminally 6His tagged Gcn4 F108A+DepositorInsertGcn4 F108A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationF108A, T121L, A141PPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED W
Plasmid#231871PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoED WDepositorInsertGcn4 ILVtoED W
Tags6xHis, TEV cleavage siteExpressionBacterialMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoAS
Plasmid#231859PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoASDepositorInsertGcn4 ILVtoAS
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL123S, I128S, V130A, V135A, L137S, I142SPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED
Plasmid#231857PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoEDDepositorInsertGcn4 ILVtoED
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL123D, I128E, V130E, V135E, L137D, I142DPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-deltaCKAR
Plasmid#222429PurposeFRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.DepositorInsertPlasma membrane delta-selective C kinase activity reporter
TagsCFP and YFPExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
Plasmid#231603PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA
Plasmid#231604PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag_codon_optimized-8xAAA
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot
Plasmid#231593PurposeExpress circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoterDepositorInsertTornado-2xALFATag-10xSunTag-Pseudoknot sequence
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SSD1-L1374
Plasmid#226278PurposePlasmid expressing the SSD1 allele from L-1374, under control of its native promoterDepositorInsertSSD1 (SSD1 Budding Yeast)
ExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG2A HRD
Plasmid#207540PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cellsDepositorInsertHaloTag flanked by human ATG2A locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAM72
Plasmid#227622PurposepETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)DepositorTagsHis-PrescissionExpressionBacterialMutation1-179 and aa 2-239, G77PPromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
TagsCFP and YFPExpressionMammalianAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM315 pACYC-His6-Rpn10[ΔUIM]
Plasmid#226348PurposeExpression of yeast Rpn10 with the UIM deletedDepositorAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only