We narrowed to 14,508 results for: SHR

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pSCL.176

Plasmid

#184997

Purpose

Express -Eco1 RNF2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

RNF2 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.179

Plasmid

#185000

Purpose

Express -Eco1 HEK4 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK4 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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E42_gRunx3_gRNA3_dTet_mTurquoise2

Plasmid

#189806

Purpose

Retroviral delivery of guide RNA against mouse Runx3

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx3_gRNA3 (Runx3 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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E42_gRunx3_gRNA2_dTet_mTurquoise2

Plasmid

#189805

Purpose

Retroviral delivery of guide RNA against mouse Runx3

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx3_gRNA2 (Runx3 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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E42_gRunx3_gRNA1_dTet_mTurquoise2

Plasmid

#189804

Purpose

Retroviral delivery of guide RNA against mouse Runx3

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx3_gRNA1 (Runx3 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUBI-RZ-Cas12j2

Plasmid

#189781

Purpose

Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA2

Plasmid

#189747

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA1

Plasmid

#189746

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b-gRNA-1

Plasmid

#184567

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

Plasmid

#168251

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v2 (opti)

Plasmid

#177250

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_2nd

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v1-hu6-sgCebpb_v1

Plasmid

#177251

Purpose

Expresses Cebpa_v1 (mU6), Cebpb_v1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v1/sgCebpb_v1

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpb_v2

Plasmid

#177252

Purpose

Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v1-hu6-sgCebpd_v1

Plasmid

#177253

Purpose

Expresses Cebpa_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v1/sgCebpd_v1

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpd_v2

Plasmid

#177254

Purpose

Expresses Cebpa_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpd_v2

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpb_v1-hu6-sgCebpd_v1

Plasmid

#177255

Purpose

Expresses Cebpb_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_v1/sgCebpd_v1

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpb_v2-hu6-sgCebpd_v2

Plasmid

#177256

Purpose

Expresses Cebpb_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_v2/sgCebpd_v2

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNT1 (opti)

Plasmid

#177237

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNT2 (opti)

Plasmid

#177238

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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