We narrowed to 5,033 results for: plasmid dna

1. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

   Plasmid

   #74101

   Purpose

   vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ku70-TC

   Plasmid

   #46955

   Purpose

   Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   XRCC6 targeting construct (XRCC6 Human)
   

   Use

   AAV; R

   Tags

   GFP-FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TDK promoter dCas9

   Plasmid

   #113657

   Purpose

   dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   TDK gene promoter and dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TDK gene promoter

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pStA1CZ

   Plasmid

   #114166

   Purpose

   Start-Stop Assembly Level 1CZ plasmid

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOK12

   Plasmid

   #49789

   Purpose

   E. coli cloning vector (KanR, low copy, blue/white selection)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pStA212

   Plasmid

   #114171

   Purpose

   Start-Stop Assembly Level 212 plasmid

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4.26-SS-136

   Plasmid

   #68744

   Purpose

   ZF6 Op -- GFP

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   ZF6 Op
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.26-SS-79

    Plasmid

    #68740

    Purpose

    ZF2 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF2 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.26-SS-78

    Plasmid

    #68747

    Purpose

    ZF9 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF9 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4.26-SS-139

    Plasmid

    #68746

    Purpose

    ZF8 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF8 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pStA1DZ

    Plasmid

    #114168

    Purpose

    Start-Stop Assembly Level 1DZ plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pStA1DE

    Plasmid

    #114169

    Purpose

    Start-Stop Assembly Level 1DE plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pStA1EZ

    Plasmid

    #114170

    Purpose

    Start-Stop Assembly Level 1EZ plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pStA223

    Plasmid

    #114172

    Purpose

    Start-Stop Assembly Level 223 plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pStA234

    Plasmid

    #114173

    Purpose

    Start-Stop Assembly Level 234 plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pStA0

    Plasmid

    #114161

    Purpose

    Start-Stop Assembly Level 0 plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pStA1AZ

    Plasmid

    #114162

    Purpose

    Start-Stop Assembly Level 1AZ plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pStA1AB

    Plasmid

    #114163

    Purpose

    Start-Stop Assembly Level 1AB plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pStA1BZ

    Plasmid

    #114164

    Purpose

    Start-Stop Assembly Level 1BZ plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pStA1BC

    Plasmid

    #114165

    Purpose

    Start-Stop Assembly Level 1BC plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pStA1CD

    Plasmid

    #114167

    Purpose

    Start-Stop Assembly Level 1CD plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pStA314

    Plasmid

    #114175

    Purpose

    Start-Stop Assembly Level 314 plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pStA313

    Plasmid

    #114174

    Purpose

    Start-Stop Assembly Level 313 plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107303

    Purpose

    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS2
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107304

    Purpose

    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS3
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.26-SS-140

    Plasmid

    #68739

    Purpose

    ZF1 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF1 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL4.26-SS-134

    Plasmid

    #68741

    Purpose

    ZF3 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF3 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL4.26-SS-80

    Plasmid

    #68742

    Purpose

    ZF4 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF4 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGL4.26-SS-135

    Plasmid

    #68743

    Purpose

    ZF5 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF5 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL4.26-SS-138

    Plasmid

    #68745

    Purpose

    ZF7 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF7 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGL4.26-SS-137

    Plasmid

    #68748

    Purpose

    ZF10 Op -- GFP

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF10 Op
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSc1

    Plasmid

    #80436

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYY62-P1int-TP901-GFPdropout

    Plasmid

    #235773

    Purpose

    Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid

    #74102

    Purpose

    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid

    #181747

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    

    Use

    Selection plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Prham-M.EcoRI-p15A-aadA

    Plasmid

    #149341

    Purpose

    expresses M.EcoRI DNA methyltransferase in E. coli

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

    Insert

    M.EcoRI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge