Addgene

Showing: 821 - 840 of 1561 results

Results per page:

Sort results by:

pLJM1-zeo-ORF24-3xFLAG

Plasmid

#130959

Purpose

Lentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS58 TIP120A 3'UTR

Plasmid

#14501

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Expression

Mutation

Promoter

hSyn

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV-CAG-GFP

Plasmid

#83279

Purpose

recombinant AAV vector packaging self-complementary GFP under the CAG promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

enhanced green fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1_WiChR_TS_mScarlet_ER

Plasmid

#195190

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Wobblia lunata inhibitory Channelrhodopsin

Use

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pITR-AAV-Rep2Cap9-GFP

Plasmid

#199744

Purpose

Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ GFP insert

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 356-736 in Cap with GFP

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIG-730pUC19-tNGFR-P2A-Coronin1A

Plasmid

#186110

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CORONIN1A

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p8327 pLIX YAP1 S127A

Plasmid

#184528

Purpose

Expression of YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S127A

Promoter

TRE promoter, Tet ON

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-p2A-CreERT2-FRT-kan-FRT

Plasmid

#108454

Purpose

This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

mCherry-p2a-CreERT2

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DBD (N208AH212R) Foxo1-WT pDNR-CMV

Plasmid

#17555

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

Foxo1-WT-DBD (Foxo1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

DNA binding-deficientChanged N208A H212R

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pblic-neo TRX1

Plasmid

#110919

Purpose

To overexpress Thioredoxin-1

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

unknown

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge