We narrowed to 26,307 results for: Vit

1. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

   Plasmid

   #201683

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-GST-4EBP1-Low GC

   Plasmid

   #69018

   Purpose

   expresses tagged 4EBP1

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   EIF4EBP1 (EIF4EBP1 Human)
   

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   lowGC content, wt is ~70% GC rich. Still, it code…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pTH-iCre-WPREpA

   Plasmid

   #213130

   Purpose

   Truncated rat TH promoter expressing iCre

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-HA-hRb-WT-T373A-S608A-S612A-puro

   Plasmid

   #212674

   Purpose

   Express tagged hRb mutant

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   T373A/S608A/S612A

   Promoter

   TRE

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA13-pHR-SFFV-HaloTag-FBL

   Plasmid

   #199444

   Purpose

   Expression of HaloTag-FBL in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   FBL (FBL Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FLAG-APEX2-MAP1LC3BK51A

   Plasmid

   #175754

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Mutation

   K51A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdEasy-EGFP-CLASP2 340-1362

   Plasmid

   #24522

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant that retains MT lattice bi…

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCAT077

   Plasmid

   #180511

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES2-hPAC-FLAG

   Plasmid

   #129494

   Purpose

   Expresses human TMEM206 tagged at the C-terminus with FLAG

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5_T7-GAP43-mScarlet3

    Plasmid

    #225932

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    GAP43-mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPLICS NU-MT P2A

    Plasmid

    #210129

    Purpose

    Detect the Nucleus-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

    Insert

    SPLICS NU-MT P2A
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-P3-IgKL-mNG

    Plasmid

    #183465

    Purpose

    To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-BLRR

    Plasmid

    #158958

    Purpose

    Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    BLRR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-MPRA-MluI-SpeI-EcoRI

    Plasmid

    #190196

    Purpose

    Empty vector for inserting MPRA libraries into AAV

    Depositor

    Hyejung Won

    Article

    McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

    Type

    Empty backbone

    Use

    AAV; Massively parallel reporter assay (mpra)

    Expression

    Mammalian

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #109048

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Has Service

    AAV9

    Insert

    eGtACR1-ST
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 416 pSG5L HA RB del22

    Plasmid

    #10721

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del22 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of exon 22 (aa738-775)

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSOX9-dTAG-mNeonGreen-V5

    Plasmid

    #194971

    Purpose

    AAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9

    Depositor

    Joanna Wysocka

    Article

    Naqvi et al Nat Genet. 2023 May;55(5):841-851. doi:

    Insert

    SRY-box transcription factor 9 (SOX9 Human)
    

    Use

    AAV

    Tags

    dTAG-mNeonGreen-V5

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only