We narrowed to 8,622 results for: PAN

1. pMEXneo-TrkB

   Plasmid

   #190203

   Purpose

   To express the Human TrkB receptor under the Moloney mouse sarcoma virus LTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkB (NTRK2 Human)
   

   Expression

   Mammalian

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule- Mj Acd A9

   Plasmid

   #197651

   Purpose

   E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

   Depositor

   Ryan Mehl

   Article

   Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

   Inserts

   Mj Acd A9 synthetase
   Methanocaldococcus jannaschii tRNA
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32A, L65D, F108L, Q109E, D158S, L162T

   Promoter

   GlnS and lpp

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHes1(467)-luc

   Plasmid

   #41723

   Depositor

   Ryoichiro Kageyama, Raphael Kopan

   Article

   Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

   Insert

   Hes1 Promoter (-467 to +46) (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter (-467 to +46)

   Promoter

   Hes1 promoter fragment (-467 to +46)

   Available Since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-hPhyBY276H-mCherry-HRasCT

   Plasmid

   #100540

   Purpose

   Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa), Y276H muta…

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-DeAct-SpvB

   Plasmid

   #89446

   Purpose

   Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

   Plasmid

   #154769

   Purpose

   plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-hPhyB-mCherry-HRasCT

   Plasmid

   #100281

   Purpose

   Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2KXIG (Tol2)-tp63:ikk1KD:GFP

   Plasmid

   #105645

   Purpose

   Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   tp63:ikk1KD:GFP (chuk Zebrafish)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p63 promoter + ikk1 (kinase domain deleted) + GFP

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac1-EcTrp-TGA

    Plasmid

    #228780

    Purpose

    aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

    Plasmid

    #172104

    Purpose

    Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-Bos TRAM Flag

    Plasmid

    #41551

    Depositor

    Kate Fitzgerald, Doug Golenbock

    Article

    Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

    Insert

    TRAM (TICAM2 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pTetON-DeAct-SpvB

    Plasmid

    #89455

    Purpose

    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK1508

    Plasmid

    #219750

    Purpose

    pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - npgA - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid

    #191093

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B and mScarlet
    

    Use

    AAV

    Tags

    H2B-V5-mScarlet

    Expression

    Mammalian

    Mutation

    The fusion protein was optimized to the Human cod…

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEXneo-TrkC

    Plasmid

    #190202

    Purpose

    To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Use

    Eucaryotic

    Expression

    Mammalian

    Promoter

    Moloney mouse sarcoma virus LTR

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2KXIG (Tol2)-tp63:ikk1C179A:GFP

    Plasmid

    #105647

    Purpose

    Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1C179A:GFP (chuk Zebrafish)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p63 promoter + ikk1C179A + GFP

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CSK_HUMAN_D0

    Plasmid

    #79683

    Purpose

    This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK (CSK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only