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DCT.VV.Orf34

Plasmid

#12722

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf34

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf36

Plasmid

#12724

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf36

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf39

Plasmid

#12727

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf39

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf44

Plasmid

#12732

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf44

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf46

Plasmid

#12734

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf46

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf47

Plasmid

#12735

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf47

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf49

Plasmid

#12737

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf49

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf54

Plasmid

#12742

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf54

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf57

Plasmid

#12745

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf57

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pKT2/CAGXSP/GFPCD63

Plasmid

#231709

Purpose

Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

Insert

CD63 (CD63 Human)

Tags

gfp

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Aequorea victoria, Mouse)

Tags

HA-Tag and pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_FLAG-Mx PylRS YA

Plasmid

#140012

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Mutation

Y126A

Promoter

EF1

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

Plasmid

#140014

Purpose

Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Mutation

Y126A, Y197F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-TMEM192-mKeima

Plasmid

#215358

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Article

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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