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MTS-TWINKLE(IDR)-mCh-CRY2olig

Plasmid

#254613

Purpose

Expresses mt-optoIDR with TWINKLE (1-170 aa) to optogenetically induce biomolecular condensates within mitochondrial matrix in living cells.

Depositor

Marina Feric

Publication

Aththawala Gedara et al Commun Biol. 2026 Apr 23. doi: 10.1038/s

Insert

TWNK (TWNK Human)

Use

Synthetic Biology

Tags

mCherry-Cry2olig

Expression

Mammalian

Available since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pRK415-mNeonGreen(inducible expression)

Plasmid

#252211

Purpose

Expresses mNeonGreen in a very low copy F-like plasmid under the inducible expression of a tac promoter within E. coli DH10Beta (for cloning)

Depositor

Cecile Fradin

Publication

Sakib et al Methods Appl Fluoresc. 2026 Sep 9;14(4).

Inserts

mNeonGreen
LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

deleted amino acids 1-3.

Promoter

LacI promoter and tac

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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TP1374

Plasmid

#252365

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody D9

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1378

Plasmid

#252366

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody D6

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1377

Plasmid

#252369

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody C10

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1420

Plasmid

#252370

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody I

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1421

Plasmid

#252371

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody II

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1425

Plasmid

#252372

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody VI

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1428

Plasmid

#252373

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody IX

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1429

Plasmid

#252374

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody X

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1431

Plasmid

#252375

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody XII

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1321

Plasmid

#252360

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody E4

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1322

Plasmid

#252361

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody F12

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1329

Plasmid

#252362

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody F9

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1330

Plasmid

#252363

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody B2

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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TP1331

Plasmid

#252364

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC nanobody D2

Tags

ALFA-sfGFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP

Plasmid

#217410

Purpose

Expresses E. coli DNA Polymerase III subunit delta' (holb) with a His-tag in bacterial cells

Depositor

Brian Kelch

Publication

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

DNA polymerase III subunit Delta' (holB E. coli)

Tags

His

Expression

Bacterial

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-DSBH pc2335

Plasmid

#201697

Purpose

Expresses of GFP-tagged mTet1-DSBH in mammalian cells

Depositor

Cristina Cardoso

Publication

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 DSBH (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB:GSlinker:SSD

Plasmid

#228535

Purpose

FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

Depositor

Roman Jerala

Publication

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

FRB:GSlinker:SSD (SKP1, SOCS2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-gibbon cGASdeltaN-HA

Plasmid

#186861

Purpose

Doxycycline-dependent expression of gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Gibbon cGAS trucation mutant (152-514 a.a.) with C-terminal HA tag (CGAS Synthetic, Hylobatidae)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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