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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A/S211A

Plasmid

#208370

Purpose

Mammalian expression of HA-tagged human PPM1H S124A/S211A

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A/S211A (PPM1H Human)

Expression

Mammalian

Mutation

S124A/S211A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A pMT2

Plasmid

#206110

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ115-133, 204-217 PPM1H

Plasmid

#207343

Purpose

Mammalian expression of HA-Δ115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_6F→A

Plasmid

#194269

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

F276A, F283A, F289A, F367A, F397A, F401A

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_CR

Plasmid

#194263

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

M322A, M323A, M336A, M337A, M339A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_IDR

Plasmid

#194264

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

M307A, M311A, M359A, M405A, M414A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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