We narrowed to 14,390 results for: nts

1. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

   Plasmid

   #167198

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E294K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

   Plasmid

   #167197

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R M173I
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

   Plasmid

   #167196

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E144K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJV126

   Plasmid

   #124250

   Purpose

   In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Promoter

   T7

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJV127

   Plasmid

   #124251

   Purpose

   In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Promoter

   T7

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJV128

   Plasmid

   #124252

   Purpose

   In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Promoter

   T7

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJV129

   Plasmid

   #124253

   Purpose

   In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Promoter

   T7

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p35S_EVDwt

   Plasmid

   #167119

   Purpose

   Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE (EVD) (AT5G17125 Mustard Weed)
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221 NLRP1 delta787-843

   Plasmid

   #166821

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   _787-843

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 Ub-NLRP1 S1213 P1214R

    Plasmid

    #166830

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Mutation

    P1214R

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    T755N

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    R726W

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

    Plasmid

    #164132

    Purpose

    Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    ciCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPK-352

    Plasmid

    #157922

    Purpose

    pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    tPcyA
    IRES
    HO1
    FD
    FNR
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    Mitochondrial Targeting Sequence, Mitochondrial T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRC/CMV-TYK2VF/PA-VSV

    Plasmid

    #139351

    Purpose

    Expression of the double mutant of TYK2-V678F-P1104A

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F-P1104A

    Promoter

    CMV

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BtGRK2[D110A K220R]-mVenus

    Plasmid

    #137779

    Purpose

    Visualization of GRK2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Bovine GRK2[D110A K220R] (GRK2 Bovine)
    

    Expression

    Mammalian

    Mutation

    D110A K220R

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126758

    Purpose

    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126759

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126753

    Purpose

    Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126754

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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