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pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Plasmid

#195550

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

Plasmid

#208554

Purpose

Lentiviral vector expressing gRNA targeting human BCL11B-E1

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B-E1(14) (BCL11B Human)

Use

Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryCXCR4-W

Plasmid

#200102

Purpose

Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR and Lentiviral

Mutation

silent mutations (c.180C>T, 438G>A, 495C>…

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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GOSR2(G144W)-mCherry

Plasmid

#209887

Purpose

Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells

Depositor

Geert van den Bogaart

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GOSR2 (GOSR2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed glycine 144 to tryptophan; this mutation …

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

Plasmid

#209775

Purpose

CRISPRi for STK3

Depositor

Michael Wehr

Publication

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Publication

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RUNX3 (RUNX3 Human)

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

Plasmid

#184549

Purpose

Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

Depositor

Gerardo Ferbeyre

Publication

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Retroviral

Tags

3xFLAG and HA tag

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg5_H

Plasmid

#146474

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Publication

Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsSmg5 (SMG5 Human)

Expression

Mammalian

Mutation

three non silent mutation A254G (K85R), T980C (L3…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Publication

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmAtx2_AE

Plasmid

#148548

Purpose

Insect Expression of DmAtx2

Depositor

Elisa Izaurralde

Insert

DmAtx2 (Atx2 Fly)

Expression

Insect

Mutation

Three silent mutations and two deletions compared…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CNTN2-Fc(DAPA)-AviTag-6xHis

Plasmid

#157016

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CNTN2 (CNTN2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA5B-Fc(DAPA)-AviTag-6xHis

Plasmid

#157021

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5B (SEMA5B Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-DCC-Fc(DAPA)-AviTag-6xHis

Plasmid

#157029

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

DCC (DCC Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

Plasmid

#156982

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTGFRN (PTGFRN Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA3E-Fc(DAPA)-AviTag-6xHis

Plasmid

#156970

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3E (SEMA3E Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-NTRK1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156823

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NTRK1 (NTRK1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-HLA-C-Fc(DAPA)-AviTag-6xHis

Plasmid

#156831

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HLA-C (HLA-C Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CRLF1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156814

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CRLF1 (CRLF1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIRPB1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156780

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPB1 (SIRPB1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SIRPB1.3-Fc(DAPA)-AviTag-6xHis

Plasmid

#156782

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPB1 (SIRPB1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge