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pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Use

Tags

GFP1-10

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

Plasmid

#86418

Purpose

ONM/ER split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP1-10-Scs2TM

Use

Tags

GFP1-10

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas C220S/C222S-IP

Plasmid

#13829

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas C220S/C222S (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C220 and C222 changed to serine residues

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-LOV2-Cyb5a

Plasmid

#233348

Purpose

Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted (Cyb5a 95-134) and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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Mini-Ii-Str_SBP-EGFP-LIMP2

Plasmid

#222324

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA4

Plasmid

#171002

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA

Plasmid

#170999

Purpose

HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[R537A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[R537A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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