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We narrowed to 4,045 results for: golden gate

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Showing: 2101 - 2150 of 4045 results

  5. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

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  6. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40131-plasmid-map-sequence-id-51019

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  8. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  11. pCHW111117

    Plasmid
    #222460
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222460-plasmid-map-sequence-id-454089

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  12. pCHW110035

    Plasmid
    #222444
    Purpose
    Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    LoxP-mCherry-stop
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222444-plasmid-map-sequence-id-454076

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  16. pSBLego

    Plasmid
    #219967
    Purpose
    The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
    Depositor
    Markus Seeger
    Article
    Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10
    Type
    Empty backbone
    Tags
    SLEHHHHHH and pelB signal sequence
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    219967-plasmid-map-sequence-id-435554

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  23. pCHW111114

    Plasmid
    #222459
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222459-plasmid-map-sequence-id-454092

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  24. pCHW111118

    Plasmid
    #222461
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber Mosaic Virus)
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222461-plasmid-map-sequence-id-454090

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  25. pCHW111113

    Plasmid
    #222458
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222458-plasmid-map-sequence-id-454086

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  27. pJB158

    Plasmid
    #202601
    Purpose
    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
    Depositor
    Athanasios Typas
    Article
    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202601-plasmid-map-sequence-id-412308

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  28. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  42. pMH183

    Plasmid
    #122856
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122856-plasmid-map-sequence-id-247655

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Showing: 2101 - 2150 of 4045 results