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pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Mouse, Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUPI hT5-S322-Cyp
Plasmid#80282PurposeExpresses human TRIM5 CypA fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-GST-Cys-M87 Spastin
Plasmid#184804PurposeBacterial expression of M87 Spastin (A2C mutation)DepositorInsertM87 Spastin (SPAST Human)
UseTagsHis6-GSTExpressionBacterialMutationA2CPromoterT7Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD4o
Plasmid#203751PurposeEncodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrCD4o (CD4 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G
Plasmid#194461PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-2P
Plasmid#203748PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27AC30A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27A, C30APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G
Plasmid#194463PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2ABFP
Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-C
Plasmid#201398PurposeExpresses C terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-N
Plasmid#201397PurposeExpresses N terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Tlr4(Lps)
Plasmid#27149DepositorInserttoll-like receptor 4(Lps) (Tlr4 Mouse)
UseTags3x FLAGExpressionMammalianMutationchanged Proline 712 to Histidine. Native signal p…PromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66-2xStrep
Plasmid#130953PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length)PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG17 IQGAP1 3'UTR
Plasmid#14503DepositorInsertIQGAP1 3'UTR (IQGAP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS50 KIF2 3'UTR mut
Plasmid#14494DepositorInsertKIF2 3'UTR mutant (KIF2A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG34 RELA 3'UTR mut
Plasmid#14506DepositorInsertRELA 3'UTR mutant (RELA Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGTGCCT’s were changed to GTCGGT'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad3
Plasmid#89828PurposeKnockdown human Smad3 expression in human mammalian cellsDepositorInsertSMAD3 shRNA (SMAD3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-P
Plasmid#203747PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only
