We narrowed to 24,925 results for: crispr

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Publication

Di Donato et al Genome Res. 2016 May;26(5):681-92. doi:

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Publication

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Promoter

gfaABC1D (astrocyte specific)

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMK243 (Tet-OsTIR1-PURO)

Plasmid

#72835

Purpose

Expresses OsTIR1 under the control of the Tet promoter

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-Tet-OsTIR1-PURO-AAVS1

Use

CRISPR

Expression

Mammalian

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pCMV-SpRY-ABE8e

Plasmid

#185671

Purpose

Expresses SpRY-ABE8e in mammalian cells

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(8e)-nSpRY

Use

CRISPR

Expression

Mammalian

Mutation

SpRY D10A

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pYPQ202

Plasmid

#86198

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance marker

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Promoter

Arabidopsis ubiquitin 10 (AtUbi10)

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDIRECT_22C

Plasmid

#91135

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Publication

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Steven Carr, Samuel Myers

Publication

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pKDsgRNA-ack

Plasmid

#62654

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

Depositor

Kristala Prather

Publication

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Publication

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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