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pLNHA-C1-HsNot3_K

Plasmid

#146771

Purpose

Mammalian Expression of HsNot3

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot3 (CNOT3 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138742

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138743

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBG1

Plasmid

#111696

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBG1 (TUBG1 Human)

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_AKIP

Plasmid

#111660

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AKIP (AURKAIP1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Δ488-592-YFP

Plasmid

#242756

Purpose

E-cadherin 4th domain full deletion

Depositor

Deborah Leckband

Article

Zou et al J Cell Sci. 2025 Nov 1;138(21):jcs264350

Insert

E-cadherin Δ488-592

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Δ525-585-YFP

Plasmid

#242757

Purpose

E-cadherin 4th domain partial deletion

Depositor

Deborah Leckband

Article

Zou et al J Cell Sci. 2025 Nov 1;138(21):jcs264350

Insert

E-cadherin 525-585 deletion

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ432:SSX1p-mK2-Ex1-(5'D)-ID-(linker1)-pA

Plasmid

#251335

Purpose

Expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ231:Inactive Control-G3BSs-TATA-mKate2-pA

Plasmid

#251314

Purpose

Inactive Control for mKate2 for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2

Expression

Mammalian

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ234:H2A1p-BD6-AS-3'SE-mK-EX2-3*miRS1-BS-pA

Plasmid

#251317

Purpose

Expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ134:CMVp-BD9-AS-(3'SE)-GAD-EX2-pA

Plasmid

#251301

Purpose

Expression of GAD-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

GAD-Ex2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ135:CMVp-GAD-EX1-(5'D)-BD9-S-pA

Plasmid

#251302

Purpose

Expression of GAD-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

GAD-Ex1

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ138:CMVp-GAD-Ex1-(5'D)-BD6-S-pA

Plasmid

#251303

Purpose

Expression of GAD-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

GAD-Ex1

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ146:CMVp-BD6-AS-(3'SE)-GAD-Ex2-pA

Plasmid

#251306

Purpose

Expression of GAD-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

GAD-Ex2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆GR pc5078

Plasmid

#229758

Purpose

Bacterial expression plasmid of MBD2∆GR. The Protein is lacking the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the glycine/arginine rich domain: aa…

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆N∆CC-G pc5089

Plasmid

#229765

Purpose

Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-C∆CC-G pc5091

Plasmid

#229766

Purpose

Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆N(MBD2b) pc4787

Plasmid

#229752

Purpose

Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the N-terminus: aa 1-152.

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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