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pACT-HAB1_star

Plasmid

#241277

Purpose

Orthogonal PYR1/HAB1 sensor module

Depositor

Sean Cutler

Publication

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

HAB1_star (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFS565

Plasmid

#242865

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus

Depositor

Nick Rhind

Publication

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFS555

Plasmid

#242855

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus

Depositor

Nick Rhind

Publication

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW26K_1Ti1

Plasmid

#177292

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

Depositor

Karl-Erich Jaeger

Publication

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW22K_7Ti1

Plasmid

#177293

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

Depositor

Karl-Erich Jaeger

Publication

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW09K_0T5

Plasmid

#177281

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

Depositor

Karl-Erich Jaeger

Publication

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Ptet-tetA(C)

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2617

Plasmid

#234496

Purpose

NATR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Publication

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

NAT-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2618

Plasmid

#234497

Purpose

NEOR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Publication

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

NEO-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2619

Plasmid

#234498

Purpose

amdS-CnU6-empty sgRNA

Depositor

Hiten Madhani

Publication

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2662

Plasmid

#234501

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdS

Depositor

Hiten Madhani

Publication

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Publication

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13BE5

Plasmid

#223836

Purpose

Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>ApacH>StayGold2

Expression

Yeast

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKS1576

Plasmid

#239524

Purpose

Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

Depositor

John Dueber

Insert

ScVba2p-mTurquoise2-2xCrk_SH3

Expression

Yeast

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Nat

Plasmid

#236494

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-G418

Plasmid

#236481

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Publication

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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