We narrowed to 13,511 results for: Mpl;
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Plasmid#157716Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
TagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-gCM
Plasmid#215868PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GEC1
Plasmid#73945PurposeFor recombinant expression of human GEC1/GABARAPL1 in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-THY1-COMP5AP-AviTag-9xHis
Plasmid#157056PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG 17xS/T->A, catalytic dead lipin 1
Plasmid#32008DepositorInsertLipin1 (Lpin1 Mouse)
TagsFLAGExpressionMammalianMutationD712E, D714E AND S106A, S150A, (S281A, T282A), S2…PromoterCMV, SP6Available SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2XStrep-ATIC
Plasmid#125685Purposeexpresses human ATIC protein with an N-terminal 2XStrep affinity tagDepositorAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #1
Plasmid#127194PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #1 (FUS Human)
TagshexahistidineExpressionBacterialMutationQQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-CPAP
Plasmid#136815PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tagDepositorInsertSNAP-CENPJ (CENPJ Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS
Plasmid#162613PurposeAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
ExpressionMammalianMutation1-155-I152LAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Elf5 gRNA
Plasmid#128836PurposegRNA for targeting mouse Elf5 loci using CRISPR-cas techniqueDepositorInsertmElf5 gRNA (Elf5 Mouse)
UseCRISPRAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-ultraID-LMNB1
Plasmid#207775PurposeDonor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep290
Plasmid#136804PurposeMammalian expression of the centrosomal protein Cep290 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep290 (CEP290 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-hDND1
Plasmid#70070PurposeGateway entry vector for human DND1 with stop codonDepositorInsertDnd1 (DND1 Human)
UseGateway entry vectorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-BFPCDK6-W
Plasmid#208756PurposeLentiviral vector expressing BFP fused with CDK6 cDNADepositorInsertCDK6 (CDK6 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3C-Fc(DAPA)-AviTag-6xHis
Plasmid#156961PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertSEMA3C (SEMA3C Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-ECT2
Plasmid#183835PurposeRepair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertECT2 homology arms with mNeonGreen-linker (ECT2 Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…Available SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
VAPA/B scFv [N479/107]
Plasmid#206742PurposeMammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.DepositorAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MEF2A-Sensor
Plasmid#208928Purposein vitro MAPK-Sensor for luminescent detection of ERK and p38-activityDepositorInsertMef2a docking sequence+Phospho site (MEF2A Human)
TagsHISx6, MBP, and SmbitExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only