We narrowed to 24,928 results for: crispr
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Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W
Plasmid#163176PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tagDepositorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Elf5 gRNA
Plasmid#128836PurposegRNA for targeting mouse Elf5 loci using CRISPR-cas techniqueDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmElf5 gRNA (Elf5 Mouse)
UseCRISPRAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4
Plasmid#123658PurposeDD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromotermU6Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4
Plasmid#123657PurposeDD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.DepositorInsertmCherry-P2A-DD-AcrIIA4
UseCRISPR, Lentiviral, and Synthetic BiologyTagsmCherry-P2AExpressionMammalianPromoterCMV and mU6Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
WT1 KI gRNA2
Plasmid#92313PurposeCRISPR-GFP-gRNA for cutting WT1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWT1 (WT1 Human)
TagsCRISPR GFPExpressionMammalianPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJGL001
Plasmid#180605PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasXDepositorInsertDpbCasX
UseCRISPRTags10x His and MBPExpressionBacterialPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-Luciferase-hvTK-bla
Plasmid#179450PurposeDonor vector to knock in firefly Luciferase C-terminal to human PER2 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseCRISPRTagsHis/FlagPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA
Plasmid#113692PurposeU6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsNLSPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)
Plasmid#114087PurposeU6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertentry vector for FnCas12a crRNAs
ExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AiP942-pX330-Sncg
Plasmid#181774PurposeCRISPR/Cas9 guide vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSncg guide
UseCRISPRExpressionMammalianAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP926-pX330-Batf3
Plasmid#181772PurposeCRISPR/Cas9 guide vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBatf3 guide
UseCRISPRExpressionMammalianAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-module1
Plasmid#222838PurposeSubcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-module2
Plasmid#222839PurposeSubcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-module3
Plasmid#222840PurposeSubcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-module4
Plasmid#222841PurposeSubcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-module5
Plasmid#222842PurposeSubcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only