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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,736 results for: LEA

Showing: 8681 - 8700 of 15736 results

  1. J-IRF5-5

    Plasmid
    #166525
    Purpose
    scFv of a human scaffold targeting Interferon regulatory factor 5. Antigen coverage aa E232-L434 of 498
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166525-plasmid-map-sequence-id-329138

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  2. J-DHTKD1A-1

    Plasmid
    #166524
    Purpose
    scFv of a human scaffold targeting Dehydrogenase E1 and transketolase domain containing protein 1. Antigen coverage aa 45-919 of 919
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166524-plasmid-map-sequence-id-329137

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  3. Z-QARS-1

    Plasmid
    #166557
    Purpose
    scFv of a human scaffold targeting Glutamine-tRNA synthetase. Antigen coverage aa 1-775 of 775, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166557-plasmid-map-sequence-id-329265

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  4. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Available Since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. 5C8H

    Plasmid
    #69969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ORC2:JMC095-D11:C231601 (ORC2 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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  7. UKC1620-SUP35wt

    Plasmid
    #29730
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Expression
    Yeast
    Mutation
    Wild type gene including genomic sequence 955 bp …
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. PP-MAPK1 (3OZ6)

    Plasmid
    #28164
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    mitogen-activated protein kinase 1 (cgd2_1960 Cryptosporidium parvum)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains residues 4-390
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

    Plasmid
    #227089
    Purpose
    Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)
    Depositor
    Chris Hill
    Insert
    tom1p-HECT (TOM1 Budding Yeast)
    Tags
    HIS-MBP-TEV_cleavage_site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227089-plasmid-map-sequence-id-451122

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  10. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

    Plasmid
    #227085
    Purpose
    Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227085-plasmid-map-sequence-id-451055

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  11. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  12. pET24a MDH_CHLP8

    Plasmid
    #204242
    Purpose
    Bacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_CHLP8
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204242-plasmid-map-sequence-id-413340

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  13. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  14. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147260-plasmid-map-sequence-id-289707

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  15. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  16. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Available Since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

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  17. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

    Plasmid
    #147279
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147279-plasmid-map-sequence-id-355936

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  18. Z-CARS-8

    Plasmid
    #166555
    Purpose
    scFv of a human scaffold targeting Cysteinyl-tRNA synthetase. Antigen coverage aa 1-748 of 748, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166555-plasmid-map-sequence-id-329263

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  19. Q-JMJD2DA-4

    Plasmid
    #166553
    Purpose
    scFv of a human scaffold targeting Lysine-specific demethylase 4D. Antigen coverage aa 1-342 of 523
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166553-plasmid-map-sequence-id-329243

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  20. T-FAM83BA-6

    Plasmid
    #166551
    Purpose
    scFv of a human scaffold targeting Protein FAM83B. Antigen coverage aa 117-294 of 1011
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166551-plasmid-map-sequence-id-329242

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Showing: 8681 - 8700 of 15736 results