We narrowed to 88,716 results for: Mal

1. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

   Plasmid

   #113886

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-Tet-On-shRab27a

   Plasmid

   #120930

   Purpose

   conditionally knockdown Rab27a in mouse cell lines

   Depositor

   Mien-Chie Hung

   Article

   Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

   Insert

   shRab27a (Rab27a Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-DIO(ChRger2-TS-YFP)

   Plasmid

   #127239

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger2-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MAP1LC3C

   Plasmid

   #137758

   Purpose

   Expression of LC3C

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   MAP1LC3C (MAP1LC3C Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p026 pCS2-EnR

   Plasmid

   #11028

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   engrailed (en Fly)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Mutation

   Engrailed repressor domain (bases encoding amino …

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-mp1

   Plasmid

   #42329

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   mp1 (LAMTOR3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-haA3A-CBE-A-NG

   Plasmid

   #220895

   Purpose

   Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-A-NG
   

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIND(V5)EcoDam

    Plasmid

    #59201

    Purpose

    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. proE-cad178-Luc

    Plasmid

    #42081

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    contains E-cadherin promoter region from -178 to …

    Promoter

    E-cadherin

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CABE-T1.2

    Plasmid

    #193258

    Purpose

    Expression of CABE-T1.2 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.2
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Talin1 head EGFP fusion

    Plasmid

    #221408

    Purpose

    Mammalian expression of Talin1 head EGFP fusion for mammalian expression

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    Talin1 head EGFP fusion (TLN1 Human)
    

    Expression

    Mammalian

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-puro Vps34-GFP

    Plasmid

    #38268

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

16. hEGFR-mEGFP

    Plasmid

    #182878

    Purpose

    Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

    Depositor

    Salvatore Chiantia

    Article

    Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

    Insert

    hEGFR-mEGFP (EGFR Human)
    

    Expression

    Mammalian

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRcCMV myc-Plk1 wt (Nigg RG6)

    Plasmid

    #41160

    Depositor

    Erich Nigg

    Article

    Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

    Insert

    Plk1 (PLK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-neo-RL-ZEB2

    Plasmid

    #35536

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB2 gene (ZEB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV14_NOP58

    Plasmid

    #73063

    Purpose

    expression clone for human NOP58 with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NOP58 ribonucleoprotein (NOP58 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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