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ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 StoL

Plasmid

#232981

Purpose

Galactose iduced expression of Gcn4 StoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++ W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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mito-CCM2-mScarlet-I

Plasmid

#208886

Purpose

Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

CCM2 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsNot3_666-753_U

Plasmid

#147722

Purpose

Bacterial Expression of HsNot3_666-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_666-753 (CNOT3 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso2-del1844-2133_T

Plasmid

#147622

Purpose

Mammalian Expression of HsNot1iso2-del1844-2133

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-del1844-2133 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-F673E_S

Plasmid

#147490

Purpose

Mammalian Expression of HsNot3-F673E

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-F673E (CNOT3 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-W390A_P

Plasmid

#147236

Purpose

Insect Expression of CeAIN2-W390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W390A

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CePAN3_1-244_P

Plasmid

#147243

Purpose

Insect Expression of CePAN3_1-244

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CePAN3_1-244

Expression

Insect

Mutation

Insertion of two AA (FQ) at position 172 compared…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CePAN3_245-632_P

Plasmid

#147244

Purpose

Insect Expression of CePAN3_245-632

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CePAN3_245-632

Expression

Insect

Mutation

one non silent mutation R349H compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsDCP1a_1-135_P

Plasmid

#147246

Purpose

Bacterial Expression of HsDCP1_1-135

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsDCP1_1-135

Expression

Bacterial

Mutation

one silent mutation T237C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1-1649_P

Plasmid

#147248

Purpose

Mammalian Expression of HsXRN1_1-1649

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsXRN1_1-1649 (XRN1 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-3xWmut_P

Plasmid

#147231

Purpose

Insect Expression of CeAIN2-3xWmut

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-3xWmut

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-W282AW366A_P

Plasmid

#147233

Purpose

Insect Expression of CeAIN2-W282AW366A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW366A

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-W282AW390A_P

Plasmid

#147234

Purpose

Insect Expression of CeAIN2-W282AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW390A

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W282AW390A_O

Plasmid

#147143

Purpose

Insect Expression of CeAIN2-W282AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW390A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W366A_O

Plasmid

#147144

Purpose

Insect Expression of CeAIN2-W366A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W366A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W366AW390A_O

Plasmid

#147145

Purpose

Insect Expression of CeAIN2-W366AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W366AW390A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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