We narrowed to 8,723 results for: Plat

1. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-KrasG13S/sgKras/Cre

   Plasmid

   #99859

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-KrasG13A/sgKras/Cre

   Plasmid

   #99855

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-KrasG13R/sgKras/Cre

   Plasmid

   #99858

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pScaffold-H1 donor

   Plasmid

   #118152

   Purpose

   PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

   Plasmid

   #112022

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha/beta into TRBC1 HDRDT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NYESO alpha into TRAC HDRT Source (pTR 223)

   Plasmid

   #112023

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NYESO beta into TRBC1 HDRT Source (pTR 277)

   Plasmid

   #112024

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta into TRBC1 HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-PDGFRb559-562del-IRES2-EGFP

    Plasmid

    #204355

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion).

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    Tags

    Expression

    Mutation

    559-562 deletion

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CMV-PDGFRb-HA

    Plasmid

    #204350

    Purpose

    Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CMV-PDGFRb559-562del-HA

    Plasmid

    #204351

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    559-562 deletion

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-HDRT-CD3z-truncCAR(anti-CD19)

    Plasmid

    #215758

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR (CD247, CD19 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RAB11A-GFP HDRT Source (pTR 143)

    Plasmid

    #112012

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-GFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RAB11A-mCherry HDRT Source (pTR 144)

    Plasmid

    #112013

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-mCherry HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CD4-C-GFP HDRT Source (pTR 152)

    Plasmid

    #112018

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-GFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CLTA-GFP HDRT Source (pTR 153)

    Plasmid

    #112016

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-GFP HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BATF-GFP HDRT Source (pTR 146)

    Plasmid

    #112015

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    BATF-GFP HDRT (BATF Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge