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pCDB179-PES-H1-L92F-Q94Y

Plasmid

#218705

Purpose

Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

PES-H1

Tags

10xHis

Expression

Bacterial

Mutation

L92F-Q94Y

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13200

Plasmid

#219875

Purpose

The base plasmid of TUNEYALI forTF10

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13245

Plasmid

#219916

Purpose

The base plasmid of TUNEYALI for TF55

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13193

Plasmid

#219868

Purpose

The base plasmid of TUNEYALI for TF03

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13223

Plasmid

#219898

Purpose

The base plasmid of TUNEYALI for TF33

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF33 (YALI1_F14650g) and homologous arm matching TF33

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13197

Plasmid

#219872

Purpose

The base plasmid of TUNEYALI forTF07

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF07 ( YALI1_D30097g) and homologous arm matching TF07

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13226

Plasmid

#219901

Purpose

The base plasmid of TUNEYALI for TF36

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13194

Plasmid

#219869

Purpose

The base plasmid of TUNEYALI for TF04

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF04 (YALI1_C02831g) and homologous arm matching TF04

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13209

Plasmid

#219884

Purpose

The base plasmid of TUNEYALI for TF19

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13208

Plasmid

#219883

Purpose

The base plasmid of TUNEYALI for TF18

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13207

Plasmid

#219882

Purpose

The base plasmid of TUNEYALI for TF17

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF17 (YALI1_B19962g) and homologous arm matching TF17

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13244

Plasmid

#219915

Purpose

The base plasmid of TUNEYALI for TF54

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF54 (YALI1_F26367g) and homologous arm matching TF54

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA332

Plasmid

#214744

Purpose

Low copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13233

Plasmid

#219906

Purpose

The base plasmid of TUNEYALI for TF43

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13206

Plasmid

#219881

Purpose

The base plasmid of TUNEYALI for TF16

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF16 (YALI1_B11759g) and homologous arm matching TF16

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13196

Plasmid

#219871

Purpose

The base plasmid of TUNEYALI forTF06

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF06 (YALI1_D34785g) and homologous arm matching TF06

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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