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pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pNPAS1-donor
Plasmid#176346PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertNPAS1 (NPAS1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSOX15-donor
Plasmid#176356PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSOX15 (SOX15 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pPRDM15-donor
Plasmid#176373PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPRDM15 (PRDM15 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZIC3-donor
Plasmid#176361PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZIC3 (ZIC3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHAP12-donor
Plasmid#176348PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTHAP12 (THAP12 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOU6F1-donor
Plasmid#176359PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPOU6F1 (POU6F1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF22-donor
Plasmid#176349PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF22 (ZNF22 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pZNF624-donor
Plasmid#132511PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF624 (ZNF624 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK521
Plasmid#72440PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseUnspecifiedTagsExpressionMutationsynthetic gene with ~40 silent substitutionsPromotern/aAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK577
Plasmid#72528PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseGene synthesisTagsExpressionMutationPromotern/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
Plasmid#219828PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRZ
Plasmid#27663DepositorTypeEmpty backboneUseRna transcriptionTagsHDV RibozymeExpressionMutationPromoterAvailable sinceApril 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2c
Plasmid#127159PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotypeDepositorInsertIghg2c (Ighg2c Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2RZ
Plasmid#27664DepositorTypeEmpty backboneUseRna transcriptionTagsHDV RibozymeExpressionMutationPromoterAvailable sinceApril 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg3
Plasmid#127160PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotypeDepositorInsertIghg3 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPATZ1-donor
Plasmid#132522PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPATZ1 (PATZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7-U4
Plasmid#111336Purposefor in vitro snRNA transcriptionDepositorInsertsnR14
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRARB-donor
Plasmid#132517PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBM16
Plasmid#45344DepositorTypeEmpty backboneUseC. glabrata expressionTagsExpressionMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT643
Plasmid#8610DepositorInsertbarstar A
UseTagsExpressionBacterialMutationTwo cysteines of barstar changed to alanine, C40A…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
T7-U1
Plasmid#111334Purposefor in vitro snRNA transcriptionDepositorInsertsnR19
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pNC891
Plasmid#20397DepositorInsertYFP-reporter
UseTagsExpressionYeastMutationX=M (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC1053
Plasmid#20406DepositorInsertCFP
UseTagsExpressionYeastMutationlinker: delta kPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC829
Plasmid#20386DepositorInsertCFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC860
Plasmid#20390DepositorInsertCFP
UseTagsExpressionYeastMutationLinker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pINER3R
Plasmid#185883PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
