We narrowed to 22,070 results for: ESC

1. mCherry-Clathrin LC-15

   Plasmid

   #55019

   Purpose

   Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin LC (CLTB Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSetB-KanR-LiLac

   Plasmid

   #184569

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Use

   Tags

   His7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-bJunVN173

   Plasmid

   #22012

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bJunVN173 (Jun Rat, Aequorea Victoria)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   c-Jun(257-338)-Venus(1-172)

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-P2X2-YFP

   Plasmid

   #22400

   Depositor

   Henry Lester

   Article

   Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

   Insert

   ATP-gated P2X channel 2 (P2rx2 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

   Plasmid

   #205022

   Purpose

   mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_STf_LamB1

    Plasmid

    #181999

    Purpose

    CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCrispr-V2-mOrange (V2mO)

    Plasmid

    #140206

    Purpose

    Lenti Crispr/Cas9 with mOrange fluorescent marker

    Depositor

    Jaffer Ajani

    Article

    Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pELF1a

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-Flavidin

    Plasmid

    #89881

    Purpose

    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K80R, K121R, K132R, K134R

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Brachyury-eGFP

    Plasmid

    #21063

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TDsmURFP-IRES-eGFP

    Plasmid

    #80344

    Purpose

    Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CMV:Flag-GpNLuc

    Plasmid

    #208840

    Purpose

    Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_CMV:Flag-GeNL

    Plasmid

    #208841

    Purpose

    Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW002-Pc-mRuby2

    Plasmid

    #111250

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mRuby2 is codon optimized for expression in P. fl…

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_CMV:Flag-OgNLuc

    Plasmid

    #208851

    Purpose

    Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF-p15ABAD- YFP

    Plasmid

    #186571

    Purpose

    p15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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