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Plasmid#71743PurposeContains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activityDepositorInsertUncoupling Protein 3 promoter (Ucp3 Mouse)
UseTagsLuciferaseExpressionBacterialMutationContains 7kb of the murine UCP3 gene encompassing…PromoterUCP3Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnI
Plasmid#124642PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombinationDepositorInsertRGECO-TnI (TNNI3 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-CD64-2
Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
UseTagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero13
Plasmid#187929PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero13DepositorInsertHero13 (BEX3 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R12DD-SCLS
Plasmid#191443Purposeexpresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397DepositorInsertHuman Talin1 R12DD (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationdeletion between residues K2375 and A2397PromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)
Plasmid#185309PurposeFor the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_D125I_47-300
UseTagsExpressionMammalianMutationD125IPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT6_SEPT7-TEV-Strep
Plasmid#174499Purposebacterial co-expression of human SEPT6 and of human SEPT7DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero20
Plasmid#187930PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero20DepositorInsertHero20 (C11orf58 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP
Plasmid#174492Purposebacterial expression of human SEPT2 fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i1-TEV-Strep
Plasmid#174500Purposebacterial co-expression of human SEPT7 and of human SEPT9_i1DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-1102 USP7
Plasmid#131246PurposeMammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses USP7 amino acids 535-1102. This truncat…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_1-560 USP7
Plasmid#131244PurposeMammalian expression of N-terminally Myc-tagged USP7, minus the UBL domainsDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses USP7 amino acids 1 - 560. This truncati…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i5-TEV-Strep
Plasmid#174502Purposebacterial co-expression of human SEPT7 and of human SEPT9_i5DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6
Plasmid#174498Purposebacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6DepositorUseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i3-TEV-Strep
Plasmid#174501Purposebacterial co-expression of human SEPT7 and of human SEPT9_i3DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS-FCER1G-1
Plasmid#109192PurposeEncodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression systemDepositorInsertHuman full-length FCER1G, codon-optimized (FCER1G Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-cPHx1
Plasmid#183674PurposePtdIns(3,4)P2 biosensor for purification from bacteriaDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsHis6-EGFPExpressionBacterialMutationAmino acids 169-329PromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-DSep1-msfGFP
Plasmid#174494Purposebacterial expression of Drosophila Sep1 fused to monomeric superfolder GFPDepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only