We narrowed to 5,331 results for: pcdna3.1

1. pcDNA3.1-TIE-V5/HIS

   Plasmid

   #205612

   Purpose

   Expression of human TIE receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TIE receptor tyrosine kinase (TIE1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1_mGFP-FGFR3-K650Q

   Plasmid

   #191756

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>C (p.K650Q) + silent mutation c.1938C&…

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)-mCherry-p115

   Plasmid

   #181996

   Purpose

   Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mCherry-p115 (ARHGEF1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CRT-GFP pcDNA3.1

   Plasmid

   #80978

   Purpose

   mammalian expression of CRT-GFP

   Depositor

   Peter Steyger

   Article

   Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

   Insert

   CRT (Calr Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   no KDEL

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-CHRFAM7A-mCherry

   Plasmid

   #62635

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Expression

   Mammalian

   Mutation

   mCherry inserted into M3-M4 loop

   Promoter

   CMV

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pY014 (pcDNA3.1-hMbCpf1)

   Plasmid

   #69986

   Purpose

   Expresses humanized MbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hMbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+) mMff isoform 5

   Plasmid

   #44601

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   mMff isoform 5 (Mff Mouse)
   

   Expression

   Mammalian

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-N-Myc_DW164AA USP7

   Plasmid

   #131252

   Purpose

   Mammalian expression of N-terminally Myc-tagged DW164AA USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 aspa…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3.1-Flag-H2A K5-9R

   Plasmid

   #63562

   Purpose

   Expression of human H2A with K5R and K9R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 5 and Lysine 9 are now Arginine

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1_FRB-GEM23-Halo

    Plasmid

    #197060

    Purpose

    Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    I3-01
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-MAP3K5 (R256C)

    Plasmid

    #47105

    Purpose

    Expresses mutant (R256C) human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 256 to cysteine

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-ccdB-Firefly

    Plasmid

    #87066

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 FLIPglu-600uDelta13V

    Plasmid

    #18014

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16A Delta13
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    F16A in binding protein

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid

    #92306

    Purpose

    Expression of humanized BsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pF154 pcDNA3.1(+)SOD1G85R

    Plasmid

    #26400

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Mutation

    G85R

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-ccdB-TwinStrep

    Plasmid

    #214988

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Tags

    TwinStrep

    Expression

    Mammalian

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge