We narrowed to 18,493 results for: Met

1. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

   Plasmid

   #112170

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   mRuby3

   Mutation

   F102G

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKAcs-GGS4X-GFP

   Plasmid

   #108583

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJP118

   Plasmid

   #176494

   Purpose

   Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Insert

   Lifeact peptide
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Promoter

   T3

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU292

   Plasmid

   #167687

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. optGluCl beta Y182F ECFP

   Plasmid

   #15105

   Depositor

   Henry Lester

   Article

   Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

   Insert

   GluCl beta (glc-2 Nematode)
   

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA, Y182F

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-RFP-cb5

   Plasmid

   #137754

   Purpose

   Targets red flourescent protein (RFP) to the endoplasmic reticulum

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   RFP-cb5
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMyosin-miRFP670nano

   Plasmid

   #127431

   Purpose

   LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-LC-Myosin
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos2-Golgi-7

    Plasmid

    #57382

    Purpose

    Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pSB1K3_pBen-sfGFP_J23101-mRFP

    Plasmid

    #128126

    Purpose

    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBen (benzoate responsive promoter)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-Cre

    Plasmid

    #47117

    Purpose

    Can be used to in vitro transcribe Cre recombinase.

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    Cre
    

    Promoter

    Lac

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. CALCOCO1 W47A-mycDDK

    Plasmid

    #137769

    Purpose

    CALCOCO1 LIR mutant

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 W47A
    

    Tags

    mycDDK

    Expression

    Mammalian

    Mutation

    W47A

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47732:PtFCP:ShBle

    Plasmid

    #104893

    Purpose

    Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    ShBle
    

    Use

    Phaeodactylum; golden gate assembly

    Tags

    PtFCP promoter and terminator

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Twist-siRNA7

    Plasmid

    #8457

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    siTwist (Twist1 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

19. Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

    Plasmid

    #135462

    Purpose

    Neuronal expression of uFLARE TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
    

    Use

    AAV

    Tags

    HA, flag, VP16

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only