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pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_F305A_S306A_H307A

Plasmid

#210793

Purpose

pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F305A_S306A_H307A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_D147N_E46Q

Plasmid

#210792

Purpose

pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D147N_E46Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT1

Plasmid

#209995

Purpose

Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

Terminator (TB1006)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS1

Plasmid

#209992

Purpose

Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

RBS (RB0034)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo-MCS

Plasmid

#212725

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-His7-NUbo-MCS

Plasmid

#212724

Purpose

Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge