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EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB

Plasmid

#236140

Purpose

Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

ZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB

Plasmid

#236142

Purpose

Plasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

ZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-FOXC1-T2A-miRFP670

Plasmid

#182336

Purpose

Expresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box C1 (FOXC1 Human)

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

Plasmid

#233368

Purpose

hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

Plasmid

#233367

Purpose

hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP

Plasmid

#233356

Purpose

CMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_off-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP

Plasmid

#233354

Purpose

CMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP

Plasmid

#233360

Purpose

CMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP

Plasmid

#233358

Purpose

CMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKC_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233355

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP

Plasmid

#233362

Purpose

CMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_AMPK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233370

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

Lentiviral

Tags

NES and mEGFP

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactiveEmpty

Plasmid

#213168

Purpose

Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgGFP

Plasmid

#213169

Purpose

Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-mock

Plasmid

#228504

Purpose

This plasmid can be used for control experiments and can also be used as a plasmid backbone

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-protein-mock

Plasmid

#228505

Purpose

This plasmid can be used for control experiments and can also be used as a plasmid backbone

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK zeta mouse

Plasmid

#224577

Purpose

Negative Control for downregulation of the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase zeta mouse (Dgkz Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBIOS1458

Plasmid

#171715

Purpose

expresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)

Depositor

Christophe Sallaud

Article

Amiour et al Commun Biol. 2021 Sep 17;4(1):1095. doi:

Insert

ZmGln1-3

Expression

Plant

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huMDA5-miR30-L1221

Plasmid

#174223

Purpose

All-in-one huMDA5 rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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