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OA-1050K (firefly luciferase)

Plasmid

#132422

Purpose

express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

firefly Luciferase gRNA array

Expression

Insect

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Expression

Yeast

Mutation

WT

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-S18

Plasmid

#127826

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_Theo-LUCID

Plasmid

#119048

Purpose

Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Publication

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Theo-LUCID_part1
Fab_Theo-LUCID_part2

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_Quin-LUCID

Plasmid

#119049

Purpose

Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Publication

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Quin-LUCID_part1
Fab_Quin-LUCID_part2

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_MTX-LUCID

Plasmid

#119047

Purpose

Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Publication

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_MTX-LUCID_part1
Fab_MTX-LUCID_part2

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Publication

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-WT

Plasmid

#114065

Purpose

protein production

Depositor

Geert Kops

Publication

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-WT (TTK Human)

Tags

GST

Expression

Insect

Mutation

none

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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DeltaNIRD KVS-1

Plasmid

#113069

Purpose

Expresses worm truncated KVS1.2 in mammalian cells

Depositor

Federico Sesti

Publication

Cai et al J Biol Chem. 2007 Jun 22;282(25):18597-6

Insert

K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)

Expression

Mammalian

Mutation

first 40 AA deletion

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MPS-3

Plasmid

#113071

Purpose

Expresses worm MPS-3 in mammalian cells

Depositor

Federico Sesti

Publication

Park et al J Biol Chem. 2005 Jun 10;280(23):21893-9

Insert

MiRP K+ channel accessory Subunit 3 (mps-3 Nematode)

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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T7T3-PacI_MafB_ISH

Plasmid

#87670

Purpose

In situ probe for mouse Mafb

Depositor

Oscar Marin

Publication

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mafb (Mafb Mouse)

Expression

Bacterial

Promoter

T7/T3

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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