We narrowed to 15,736 results for: LEA

1. pHAT4

   Plasmid

   #112585

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SMYD3

   Plasmid

   #32048

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SMYD3 (SMYD3 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. SPIN2B (SPIN2BA-c011)

   Plasmid

   #98238

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SPIN2BA (SPIN2B Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. UBC64

   Plasmid

   #25143

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubc64.248.414 (UBE2Q1 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   contains UBC domain; residues 248-414

   Available Since

   Oct. 31, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET28a MDH_PFALCI

   Plasmid

   #204235

   Purpose

   Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTagRFP-T2-LaminB1-10

   Plasmid

   #58040

   Purpose

   Localization: Nuclear Envelope, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. 21 pCOLI_A_N-GST_C_STREP

   Plasmid

   #160258

   Purpose

   N-term GST 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII and GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSETA_ionRE

   Plasmid

   #172926

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorRE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

   Plasmid

   #50417

   Purpose

   Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.

   Depositor

   Andrew Scharenberg

   Inserts

   eGFP with I-Sce I TS
   +3 mCherry
   

   Use

   Lentiviral

   Tags

   T2A dislinker and iRFP

   Expression

   Mammalian

   Mutation

   embedded I-Sce I TS from 163-185

   Promoter

   SFFV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mVenus-LaminB1-10

    Plasmid

    #56616

    Purpose

    Localization: Nuclear Envelope, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. 22 pCOLI_A_N-MBP_C_STREP

    Plasmid

    #160164

    Purpose

    N-term MBP 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII, 6xHis, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mKO2-LaminB1-10

    Plasmid

    #57878

    Purpose

    Localization: Nuclear Envelope, Excitation: 551, Emission: 565

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mKO2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. tdTurboRFP-LaminB1-10

    Plasmid

    #58060

    Purpose

    Localization: Nuclear Envelope, Excitation: 553, Emission: 576

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    tdTurboRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pQUAST-nucLacZ

    Plasmid

    #24356

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    LacZ (lacZ )
    

    Tags

    8 aa nuclear localization signal

    Expression

    Insect

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 5J3E (THYN1)

    Plasmid

    #79524

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    THYN1 (THYN1 Human)
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pT7-V5-SBP-C1-HsMex3C_225-489_AD

    Plasmid

    #148498

    Purpose

    Mammalian Expression of HsMex3C_226-489. *Please note that this expresses amino acids 226-489 (not 225 as the title suggests).

    Depositor

    Elisa Izaurralde

    Insert

    HsMex3C_226-489 (MEX3C Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mKO-LaminB1-10

    Plasmid

    #57846

    Purpose

    Localization: Nuclear Envelope, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. WNK3 (WNK3B-c016)

    Plasmid

    #110252

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK3 (WNK3 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid

    #125207

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter

    Depositor

    Eric Davis

    Insert

    LPAR_AKAR-mut
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2iC6Dronpa2nlsER4(#636)

    Plasmid

    #184067

    Purpose

    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Dronpa2-2nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge