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pCS105

Plasmid

#174830

Purpose

Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

Depositor

Carol Greider

Article

Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

Insert

11R Unique

Tags

GAL4 5XUAS

Expression

Bacterial

Mutation

Changing TEL11R to a unique end

Promoter

lac promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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px330 WAPL gRNA

Plasmid

#165595

Purpose

Insertion of WAPL degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

WAPL (Wapl Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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px330 BRM gRNA

Plasmid

#165586

Purpose

Insertion of BRM degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRM (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V

Plasmid

#163876

Purpose

Acute Depletion of BRM-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brm (Smarca2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2952

Plasmid

#135586

Purpose

SNR6 has UUC at position 105–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ3 has UUC at position 105–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2953

Plasmid

#135587

Purpose

SNR6 has AUUUC at position 103–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ5 has AUUUC at position 103–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2955

Plasmid

#135589

Purpose

SNR6 has UUUAUUUC at position 100–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ8 has UUUAUUUC at position 100–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 396

Plasmid

#138063

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23110- pSEVA-331Bb- RFP

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab23-DN

Plasmid

#109734

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab23

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab23 (rab23 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab23

Plasmid

#109680

Purpose

Multisite gateway vector for 3' tagging with rab23

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab23 (rab23 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab23

Plasmid

#109612

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab23

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab23 (rab23 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet139

Plasmid

#121913

Purpose

Charge-Introduced NpuDnaB mini-intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 21-55 eGFP SV40

Plasmid

#132653

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.178

Plasmid

#119645

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS3 Down Flank

Use

Synthetic Biology

Mutation

4 nucleotide insertion between nt 65 and 66 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 41-55 eGFP SV40

Plasmid

#132652

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.176

Plasmid

#119644

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Down Flank

Use

Synthetic Biology

Mutation

bp 302 T to C

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.175

Plasmid

#119643

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Up Flank

Use

Synthetic Biology

Mutation

bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.174

Plasmid

#119641

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS1 Down Flank

Use

Synthetic Biology

Mutation

bp 658 G to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.171

Plasmid

#119639

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Up Flank

Use

Synthetic Biology

Mutation

A691T in NS4 Up Flank

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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